The aim of this course is to provide to students the insights into the application of animal and plant tissue culture. It involves correlation with practical course to ensure full understanding of the techniques used in tissue culture and help the students to understand how to develop micro-environment and which factors will affect tissue culture, media formulations, cell lines and different applications of tissue culture.
Indicative Content:
Plant tissue culture
1.Introduction ( History).
2. Preparation and composition of nutrient media.
3.Care of Plant material .
4.Isolation, inoculation and subculturing.
5.Transfer from nutrient medium to soil .
6.Embryon culture .
7.Production of disease free plants.
8.Vegetative propagation.
9.Somaclonal variation.
Animal Tissue Culture
- Origin and principles of cell culture
- In vitro animal tissue culture
- Culture medium formulation, Mammalian cell culture; Stem cell culture, Qualitative characteristics of cell cultures, cell counting and analysis, Primary cell culture techniques, Subculture and Development of immortalized cell line, Detection of microbial contaminants
- Advantages and limitations of animal tissue culture
- Cryopreservation and Cell banking. Applications of tissue culture
The module aims to provide to the students the fundamental and practical insights to exploit enzymes and microbes for the manufacturing of products which have a huge industrial significance. It uniquely blends the science and engineering with various biochemical processes to obtain products of diverse fields such as chemicals, food, bioenergy, etc. The module introduces bioreactors, its types, operation methods and provides an experimental demonstration of the same. Strategies to obtain higher yields, design of the bioreactors and production of goods for industry, including chemicals, plastics, food, agricultural and pharmaceutical products and energy carriers. The module majorly focuses on the applications and allows students to gain practical knowledge rather than mere theory. Thus, the graduates will be able to enter industry with an appropriate level of understanding of the need for both the science and business aspects to be achievable to make a viable product.
The aim of this module is to enable students to acquire comprehensive knowledge of environmental biotechnological processes with a focus on bioremediation, phytoremediation, biofuel formation, biofertilization, biopesticides, solid waste management, biomarkers and biosensors.
Dr. Diane Umuhoza